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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
16/07/2020 |
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Data da última atualização: |
10/08/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
DIAS, J. H.; PUPIN, M. A.; DUARTE, G. S.; BRAIR, V. L.; PAULA, C. J. C. de; SOUSA, M. A. P. de; BATISTA, R. I. T. P.; SOUZA-FABJAN, J. G. S.; OLIVEIRA, M. E. F.; FONSECA, J. F. da. |
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Afiliação: |
JENNIFFER HAUSCHILDT DIAS, Universidade Federal de Viçosa; MARIA AMÉLIA PUPIN, Universidade Estadual Paulista; GABRIELA SALONI DUARTE, Universidade Estadual Paulista; VIVIANE LOPES BRAIR, Universidade Federal Fluminense; CLEBER JONAS CARVALHO DE PAULA, Universidade Federal Fluminense; MARCO ANTONIO PAULA DE SOUSA, Universidade Federal do Pará; RIBRIO IVAN TAVARES PEREIRA BATISTA, Universidade Federal Fluminense; JOANNA MARIA GONÇALVES SOUZA-FABJAN, Universidade Federal Fluminense; MARIA EMÍLIA FRANCO OLIVEIRA, Universidade Estadual Paulista; JEFERSON FERREIRA DA FONSECA, CNPC. |
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Título: |
Successful transcervical uterine flushing can be performed without or reduced dose of oestradiol benzoate in cervical relaxation protocol in Dorper ewes. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Reproduction in Domestic Animals, v. 55, n. 7, p. 844-850, Jul. 2020. |
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DOI: |
https://doi.org/10.1111/rda.13692 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: This study assessed the efficiency of cervical relaxation protocol using none, half or full dose (1.0 mg) of oestradiol benzoate in Dorper ewes subjected to non?surgical embryo recovery (NSER). Thirty?six pluriparous ewes received progestogen sponge (60 mg) for 9 days plus eCG administration (300 IU i.m.) 24 hr before sponge removal. Ewes were not mated and were randomly assigned to receive at 16 hr before NSER 37.5 µg d?cloprostenol i.m. and different doses of oestradiol benzoate: 0.0 mg (0EB group; n = 12); 0.5 mg (0.5EB group; n = 12) or 1.0 mg of oestradiol (1.0EB group, n = 12). All ewes received oxytocin (50 IU) i.v. 20 min before NSER, which was performed 8 days after sponge removal. Corpora lutea were counted by transrectal ultrasonography 24 hr before NSER. After procedure, the ewes were kept in natural breeding period to check their post?NSER fertility. NSER was performed in 91.7% (33/36) of the animals with overall fluid recovery efficiency over 97% (p > .05). The cervical transposing with Hegar dilator was longer (p < .05) in 0EB (4.2 ± 0.3 min) compared to 0.5EB (1.7 ± 0.3 min) and 1.0EB group (1.5 ± 0.3 min). The cervical transposing with mandrel/catheter was longer (p < .05) in 0EB (2.4 ± 0.5 min) than 1.0EB group (1.3 ± 0.5 min). Overall duration of uterine flushing was 25.4 min with structure recovery rate of 43.5%, with no difference among groups (p > .05). The post?NSER fertility was higher (p < .05) in 0.0EB (90%) than 0.5EB group (36.4%). In conclusion, NSER can be successfully performed in Dorper ewes by using a cervical relaxation protocol without oestradiol benzoate. MenosAbstract: This study assessed the efficiency of cervical relaxation protocol using none, half or full dose (1.0 mg) of oestradiol benzoate in Dorper ewes subjected to non?surgical embryo recovery (NSER). Thirty?six pluriparous ewes received progestogen sponge (60 mg) for 9 days plus eCG administration (300 IU i.m.) 24 hr before sponge removal. Ewes were not mated and were randomly assigned to receive at 16 hr before NSER 37.5 µg d?cloprostenol i.m. and different doses of oestradiol benzoate: 0.0 mg (0EB group; n = 12); 0.5 mg (0.5EB group; n = 12) or 1.0 mg of oestradiol (1.0EB group, n = 12). All ewes received oxytocin (50 IU) i.v. 20 min before NSER, which was performed 8 days after sponge removal. Corpora lutea were counted by transrectal ultrasonography 24 hr before NSER. After procedure, the ewes were kept in natural breeding period to check their post?NSER fertility. NSER was performed in 91.7% (33/36) of the animals with overall fluid recovery efficiency over 97% (p > .05). The cervical transposing with Hegar dilator was longer (p < .05) in 0EB (4.2 ± 0.3 min) compared to 0.5EB (1.7 ± 0.3 min) and 1.0EB group (1.5 ± 0.3 min). The cervical transposing with mandrel/catheter was longer (p < .05) in 0EB (2.4 ± 0.5 min) than 1.0EB group (1.3 ± 0.5 min). Overall duration of uterine flushing was 25.4 min with structure recovery rate of 43.5%, with no difference among groups (p > .05). The post?NSER fertility was higher (p < .05) in 0.0EB (90%) than 0.5EB group (36.4%). In co... Mostrar Tudo |
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Palavras-Chave: |
Cervical dilation; NSER; Post-NSER fertility; Transcervical embryo recovery. |
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Thesaurus Nal: |
Animal reproduction; Sheep. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02663naa a2200313 a 4500
001 2123886
005 2021-08-10
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1111/rda.13692$2DOI
100 1 $aDIAS, J. H.
245 $aSuccessful transcervical uterine flushing can be performed without or reduced dose of oestradiol benzoate in cervical relaxation protocol in Dorper ewes.$h[electronic resource]
260 $c2020
520 $aAbstract: This study assessed the efficiency of cervical relaxation protocol using none, half or full dose (1.0 mg) of oestradiol benzoate in Dorper ewes subjected to non?surgical embryo recovery (NSER). Thirty?six pluriparous ewes received progestogen sponge (60 mg) for 9 days plus eCG administration (300 IU i.m.) 24 hr before sponge removal. Ewes were not mated and were randomly assigned to receive at 16 hr before NSER 37.5 µg d?cloprostenol i.m. and different doses of oestradiol benzoate: 0.0 mg (0EB group; n = 12); 0.5 mg (0.5EB group; n = 12) or 1.0 mg of oestradiol (1.0EB group, n = 12). All ewes received oxytocin (50 IU) i.v. 20 min before NSER, which was performed 8 days after sponge removal. Corpora lutea were counted by transrectal ultrasonography 24 hr before NSER. After procedure, the ewes were kept in natural breeding period to check their post?NSER fertility. NSER was performed in 91.7% (33/36) of the animals with overall fluid recovery efficiency over 97% (p > .05). The cervical transposing with Hegar dilator was longer (p < .05) in 0EB (4.2 ± 0.3 min) compared to 0.5EB (1.7 ± 0.3 min) and 1.0EB group (1.5 ± 0.3 min). The cervical transposing with mandrel/catheter was longer (p < .05) in 0EB (2.4 ± 0.5 min) than 1.0EB group (1.3 ± 0.5 min). Overall duration of uterine flushing was 25.4 min with structure recovery rate of 43.5%, with no difference among groups (p > .05). The post?NSER fertility was higher (p < .05) in 0.0EB (90%) than 0.5EB group (36.4%). In conclusion, NSER can be successfully performed in Dorper ewes by using a cervical relaxation protocol without oestradiol benzoate.
650 $aAnimal reproduction
650 $aSheep
653 $aCervical dilation
653 $aNSER
653 $aPost-NSER fertility
653 $aTranscervical embryo recovery
700 1 $aPUPIN, M. A.
700 1 $aDUARTE, G. S.
700 1 $aBRAIR, V. L.
700 1 $aPAULA, C. J. C. de
700 1 $aSOUSA, M. A. P. de
700 1 $aBATISTA, R. I. T. P.
700 1 $aSOUZA-FABJAN, J. G. S.
700 1 $aOLIVEIRA, M. E. F.
700 1 $aFONSECA, J. F. da
773 $tReproduction in Domestic Animals$gv. 55, n. 7, p. 844-850, Jul. 2020.
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Embrapa Caprinos e Ovinos (CNPC) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Cerrados. Para informações adicionais entre em contato com cpac.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
25/05/2009 |
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Data da última atualização: |
24/02/2010 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
OLIVEIRA-FILHO, E. C.; GRISOLIA, C. K.; PAUMGARTTEN, F. J. R. |
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Afiliação: |
Eduardo Cyrino Oliveira-Filho, CPAC; Cesar Koppe Grisolia, UnB; Francisco José Roma Paumgartten, Fiocruz. |
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Título: |
Effects of endosulfan and ethanol on the reproduction of the snail Biomphalaria tenagophila: a multigeneration study. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Chemosphere, v. 75, p. 398-404, 2009. |
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Idioma: |
Inglês |
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Conteúdo: |
Endosulfan (END) is an insecticide used in agriculture and as a wood preservative. Since END is practically insoluble in water, ethanol (ETOH) is often employed as a carrier solvent to spike in the test medium in aquatic toxicity assays. In this study were investigated the effects of END and ETOH on the reproduction of the freshwater snail Biomphalaria tenagophila exposed over three successive generations. END (0, 0.001, 0.01, 0.1 mg l -1) was dissolved in the medium water using ETOH (up to 19.8 mg L -1) as carrier solvent. ETOH (19.8, 198, 1980 mg L -1) alone was tested as well. Adult snails (Fo-generation) were exposed to END and ETOH for 8 weeks. The F1-generation continued to be exposed from embryo to reproductive maturity, while their descendants (F2) were exposed until day 10 after spawning. Effects on the fecundity (8-week production of eggs and egg-masses) of mature Fo and F1 snails were evaluated. Developmental toxicity was investigated in F1, and F2 embryos. END at the highest level tested (0.1 mg L -1) inhibited egg production by Fo and F1 snails. ETOH at levels >=198 mg L -1 also reduced fecundity of Fo and F1, an effect that was apparently aggravated by exposure over successive generations. END 0.1 mh L 1- increased mortality and malformations and decreased hatching among F1 embryos. ETOH drastically reduced the proportion of hatchings among F2 embryos. The study-derived NOECs (no-observed-effect-concentrations) for END was 0.01 mg L -1 (reduction in fecundity), and for ETOH were 19.8 mh L -1 for reduction in fecundity and <19.8 mg L -1 for developmental toxicity (hatching retardation). MenosEndosulfan (END) is an insecticide used in agriculture and as a wood preservative. Since END is practically insoluble in water, ethanol (ETOH) is often employed as a carrier solvent to spike in the test medium in aquatic toxicity assays. In this study were investigated the effects of END and ETOH on the reproduction of the freshwater snail Biomphalaria tenagophila exposed over three successive generations. END (0, 0.001, 0.01, 0.1 mg l -1) was dissolved in the medium water using ETOH (up to 19.8 mg L -1) as carrier solvent. ETOH (19.8, 198, 1980 mg L -1) alone was tested as well. Adult snails (Fo-generation) were exposed to END and ETOH for 8 weeks. The F1-generation continued to be exposed from embryo to reproductive maturity, while their descendants (F2) were exposed until day 10 after spawning. Effects on the fecundity (8-week production of eggs and egg-masses) of mature Fo and F1 snails were evaluated. Developmental toxicity was investigated in F1, and F2 embryos. END at the highest level tested (0.1 mg L -1) inhibited egg production by Fo and F1 snails. ETOH at levels >=198 mg L -1 also reduced fecundity of Fo and F1, an effect that was apparently aggravated by exposure over successive generations. END 0.1 mh L 1- increased mortality and malformations and decreased hatching among F1 embryos. ETOH drastically reduced the proportion of hatchings among F2 embryos. The study-derived NOECs (no-observed-effect-concentrations) for END was 0.01 mg L -1 (reduction in fecundity),... Mostrar Tudo |
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Palavras-Chave: |
Ecotoxicologia. |
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Thesagro: |
Inseticida. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02144naa a2200169 a 4500
001 1558319
005 2010-02-24
008 2009 bl --- 0-- u #d
100 1 $aOLIVEIRA-FILHO, E. C.
245 $aEffects of endosulfan and ethanol on the reproduction of the snail Biomphalaria tenagophila$ba multigeneration study.
260 $c2009
520 $aEndosulfan (END) is an insecticide used in agriculture and as a wood preservative. Since END is practically insoluble in water, ethanol (ETOH) is often employed as a carrier solvent to spike in the test medium in aquatic toxicity assays. In this study were investigated the effects of END and ETOH on the reproduction of the freshwater snail Biomphalaria tenagophila exposed over three successive generations. END (0, 0.001, 0.01, 0.1 mg l -1) was dissolved in the medium water using ETOH (up to 19.8 mg L -1) as carrier solvent. ETOH (19.8, 198, 1980 mg L -1) alone was tested as well. Adult snails (Fo-generation) were exposed to END and ETOH for 8 weeks. The F1-generation continued to be exposed from embryo to reproductive maturity, while their descendants (F2) were exposed until day 10 after spawning. Effects on the fecundity (8-week production of eggs and egg-masses) of mature Fo and F1 snails were evaluated. Developmental toxicity was investigated in F1, and F2 embryos. END at the highest level tested (0.1 mg L -1) inhibited egg production by Fo and F1 snails. ETOH at levels >=198 mg L -1 also reduced fecundity of Fo and F1, an effect that was apparently aggravated by exposure over successive generations. END 0.1 mh L 1- increased mortality and malformations and decreased hatching among F1 embryos. ETOH drastically reduced the proportion of hatchings among F2 embryos. The study-derived NOECs (no-observed-effect-concentrations) for END was 0.01 mg L -1 (reduction in fecundity), and for ETOH were 19.8 mh L -1 for reduction in fecundity and <19.8 mg L -1 for developmental toxicity (hatching retardation).
650 $aInseticida
653 $aEcotoxicologia
700 1 $aGRISOLIA, C. K.
700 1 $aPAUMGARTTEN, F. J. R.
773 $tChemosphere$gv. 75, p. 398-404, 2009.
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